We apply an integrative approach to visualize and understand dynamic protein/membrane assemblies in action, with the goal of understanding the molecular basis for cell morphology. We seek to understand protein behavior in situ.

Structural Biology

We use electron cryo-microscopy (Cryo-EM) and X-ray crystallography to understand the structure of protein machines. Together with other biophysical methods, we are interested in understanding protein motion (conformational change) as these assemblies undergo their mechanical cycle.

credit image: pdb 3KK8

​In vitro reconstitution

​We use synthetic membrane systems to dissect molecular mechanism. We reconstitute protein assembly and activity in near native liposome and supported bilayer systems. Our goal is to recapture essential elements of membrane dynamics and structure from purified components to understand native organelle function.

​credit image: pdb 6GCI, 6B8H, Fawcett The Cell

New Tools

We're interested in collaborations that leverage small molecule and protein-based tools to understand protein conformational change in cells. We explore new approaches to visualize in situ cell morphology, to understand protein conformational state and dynamics in native contexts.

credit ​image: pdb 3SOA